Scout-MRM: Multiplexed Targeted Mass Spectrometry-Based Assay without Retention Time Scheduling Exemplified by Dickeya dadantii Proteomic Analysis during Plant Infection.
Identifieur interne : 000396 ( Main/Exploration ); précédent : 000395; suivant : 000397Scout-MRM: Multiplexed Targeted Mass Spectrometry-Based Assay without Retention Time Scheduling Exemplified by Dickeya dadantii Proteomic Analysis during Plant Infection.
Auteurs : Blandine Rougemont [France] ; Sébastien Bontemps Gallo [France] ; Sophie Ayciriex [France] ; Romain Carrière [France] ; Hubert Hondermarck [Australie] ; Jean Marie Lacroix [France] ; J C Yves Le Blanc [Canada] ; Jérôme Lemoine [France]Source :
- Analytical chemistry [ 1520-6882 ] ; 2017.
Abstract
Targeted mass spectrometry of a surrogate peptide panel is a powerful method to study the dynamics of protein networks, but chromatographic time scheduling remains a major limitation for dissemination and implementation of robust and large multiplexed assays. We unveil a Multiple Reaction Monitoring method (Scout-MRM) where the use of spiked scout peptides triggers complex transition lists, regardless of the retention time of targeted surrogate peptides. The interest of Scout-MRM method regarding the retention time independency, multiplexing capability, reproducibility, and putative interest in facilitating method transfer was illustrated by a 782-peptide-plex relative assay targeting 445 proteins of the phytopathogen Dickeya dadantii during plant infection.
DOI: 10.1021/acs.analchem.6b03201
PubMed: 28029036
Affiliations:
- Australie, Canada, France
- Auvergne-Rhône-Alpes, Hauts-de-France, Nord-Pas-de-Calais, Rhône-Alpes
- Lille, Villeurbanne
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France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Villeurbanne</settlement></placeName></affiliation></author><author><name sortKey="Carriere, Romain" sort="Carriere, Romain" uniqKey="Carriere R" first="Romain" last="Carrière">Romain Carrière</name><affiliation wicri:level="3"><nlm:affiliation>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, Université Claude Bernard Lyon 1, Ecole 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V8</wicri:regionArea><wicri:noRegion>Ontario L4K 4 V8</wicri:noRegion></affiliation></author><author><name sortKey="Lemoine, Jerome" sort="Lemoine, Jerome" uniqKey="Lemoine J" first="Jérôme" last="Lemoine">Jérôme Lemoine</name><affiliation wicri:level="1"><nlm:affiliation>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne</wicri:regionArea><wicri:noRegion>69100 Villeurbanne</wicri:noRegion><wicri:noRegion>69100 Villeurbanne</wicri:noRegion></affiliation></author></analytic><series><title level="j">Analytical chemistry</title><idno type="eISSN">1520-6882</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Targeted mass spectrometry of a surrogate peptide panel is a powerful method to study the dynamics of protein networks, but chromatographic time scheduling remains a major limitation for dissemination and implementation of robust and large multiplexed assays. We unveil a Multiple Reaction Monitoring method (Scout-MRM) where the use of spiked scout peptides triggers complex transition lists, regardless of the retention time of targeted surrogate peptides. The interest of Scout-MRM method regarding the retention time independency, multiplexing capability, reproducibility, and putative interest in facilitating method transfer was illustrated by a 782-peptide-plex relative assay targeting 445 proteins of the phytopathogen Dickeya dadantii during plant infection.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Canada</li><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Hauts-de-France</li><li>Nord-Pas-de-Calais</li><li>Rhône-Alpes</li></region><settlement><li>Lille</li><li>Villeurbanne</li></settlement></list><tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Rougemont, Blandine" sort="Rougemont, Blandine" uniqKey="Rougemont B" first="Blandine" last="Rougemont">Blandine Rougemont</name></region><name sortKey="Ayciriex, Sophie" sort="Ayciriex, Sophie" uniqKey="Ayciriex S" first="Sophie" last="Ayciriex">Sophie Ayciriex</name><name sortKey="Bontemps Gallo, Sebastien" sort="Bontemps Gallo, Sebastien" uniqKey="Bontemps Gallo S" first="Sébastien" last="Bontemps Gallo">Sébastien Bontemps Gallo</name><name sortKey="Carriere, Romain" sort="Carriere, Romain" uniqKey="Carriere R" first="Romain" last="Carrière">Romain Carrière</name><name sortKey="Lacroix, Jean Marie" sort="Lacroix, Jean Marie" uniqKey="Lacroix J" first="Jean Marie" last="Lacroix">Jean Marie Lacroix</name><name sortKey="Lemoine, Jerome" sort="Lemoine, Jerome" uniqKey="Lemoine J" first="Jérôme" last="Lemoine">Jérôme Lemoine</name></country><country name="Australie"><noRegion><name sortKey="Hondermarck, Hubert" sort="Hondermarck, Hubert" uniqKey="Hondermarck H" first="Hubert" last="Hondermarck">Hubert Hondermarck</name></noRegion></country><country name="Canada"><noRegion><name sortKey="Le Blanc, J C Yves" sort="Le Blanc, J C Yves" uniqKey="Le Blanc J" first="J C Yves" last="Le Blanc">J C Yves Le Blanc</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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